1D, 2D NMR and various Spectroscopic Techniques for the Structural Characterisation of A2 Cow Colostrum Oligosaccharides

Abstract: Cow milk is one of the most widely accessible types of milk worldwide. The milk that mammals produce in the first few days after childbirth is called colostrum. A modified method of Kobata and Ginsburg including deproteination, microfiltration and lyophilization was used to process the milk of the A2 cow. This led to the isolation of crude milk oligosaccharide mixture. The general methods of purification (gel filtration and column chromatography) and structure elucidation (¹H, ¹³C, HMBC, HSQC, TOCSY and COSY) of the oligosaccharide found in the A2 cow colostrum have been incorporated in this paper.

Keywords: Milk oligosaccharides, Kobata and Ginsburg, A2 cow colostrum, Structure elucidation, 1D-2D NMR.