Green synthesis of silver nanoparticles using Citrus Orange peels and evaluation of their anticancer Property

Abstract: This work aimed to synthesis silver nanoparticles (AgNPs) using biological waste products Citrus orange peels, its characterization, and their cytotoxic effect. SEM and TEM analysis confirmed the formation of spherical and uninform nanoparticle.  The average size of the synthesized nanoparticle was 10-11 nm as measured by DLS technique. FTIR analysis of the green synthesized AgNPs showed the presence of alcohols, phenolics, alkynes, aliphatic primary amines, and amino acid groups. Finally, the evaluation of the cytotoxic effect of the green synthesized AgNPs were done using human colon carcinoma cell line (HCT-116). The results revealed the concentration has a direct correlation with cell viability. The 50% inhibitory concentration (IC50) of the HCT-116 cell line was expected as 8-10 μg/ml.

Keyword: Citrus orange peel, silver nanoparticle, characterisation, anticancer agent